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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIQUEIRA, L. G. B.; HANSEN, P. J. |
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Afiliação: |
LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; PETER J. HANSEN, University of Florida, Gainesville, Florida, USA. |
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Título: |
Sex differences in response of the bovine embryo to colony-stimulating factor 2. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Reproduction, n. 152, p. 645-654, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
We tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine. MenosWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results a... Mostrar Tudo |
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Palavras-Chave: |
CSF2; Embryokine. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/154808/1/Cnpgl-2016-Reproduction-Sex.pdf
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Marc: |
LEADER 02045naa a2200157 a 4500
001 2062824
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aSIQUEIRA, L. G. B.
245 $aSex differences in response of the bovine embryo to colony-stimulating factor 2.$h[electronic resource]
260 $c2016
520 $aWe tested whether gene expression of the bovine morula is modified by CSF2 in a sex-dependent manner and if sex determines the effect of CSF2 on competence of embryos to become blastocysts. Embryos were produced in vitro using X- or Y-sorted semen and treated at Day 5 of culture with 10 ng/mL bovine CSF2 or control. In experiment 1, morulae were collected at Day 6 and biological replicates (n = 8) were evaluated for transcript abundance of 90 genes by RT-qPCR using the Fluidigm Delta Gene assay. Expression of more than one-third (33 of 90) of genes examined was affected by sex. The effect of CSF2 on gene expression was modified by sex (P < 0.05) for five genes (DDX3Y/DDX3X-like, NANOG, MYF6, POU5F1 and RIPK3) and tended (P < 0.10) to be modified by sex for five other genes (DAPK1, HOXA5, PPP2R3A, PTEN and TNFSF8). In experiment 2, embryos were treated at Day 5 with control or CSF2 and blastocysts were collected at Day 7 for immunolabeling to determine the number of inner cell mass (ICM) and trophectoderm (TE) cells. CSF2 increased the percent of putative zygotes that became blastocysts for females, but did not affect the development of males. There was no effect of CSF2 or interaction of CSF2 with sex on the total number of blastomeres in blastocysts or in the number of inner cell mass or trophectoderm cells. In conclusion, CSF2 exerted divergent responses on gene expression and development of female and male embryos. These results are evidence of sexually dimorphic responses of the preimplantation embryo to this embryokine.
653 $aCSF2
653 $aEmbryokine
700 1 $aHANSEN, P. J.
773 $tReproduction$gn. 152, p. 645-654, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 15 | |
| 1. |  | VILLARREAL NUNEZ, J. E.; AMARAL SOBRINHO, N. M. B. do; KUNSMANN, M.; PALMIERI, F. Influencia do tipo de preparo do solo sobre as formas de fosforo em um solo tipico da Microbacia de Caetes (Paty do Alferes, RJ). In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 26., 1997, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: Sociedade Brasileira de Ciencia do Solo, 1997. p.53.| Biblioteca(s): Embrapa Solos. |
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| 4. | | ARAUJO, W. S. A. de; AMARAL SOBRINHO, N. M. B. do; MAZUR, N.; GOMES, P. C. Relação entre adsorção de metais pesados e atributos químicos e físicos das principais classes de solo do Brasil. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 27., 1999, Brasília, DF. Resumos [...]. Planaltina, DF: Embrapa Cerrados, 1999. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 5. | | VILLAREAL NUNEZ, J. E.; AMARAL SOBRINHO, N. M. B. do; KUNZMANN, M.; PALMIERI, F. Perdas de fosforo por erosao afetado pelo metodo de preparo do solo na microbacia de Caetes, Paty do Alferes, RJ. In: WORKSHOP NACIONAL DE AGRICULTURA SUSTENTAVEL EM REGIOES DE RELEVO ACIDENTADO, 3., 1997. Paty do Alferes, RJ. Anais... Rio de Janeiro: EMBRAPA-CNPS, 1998. CD-ROM Nao paginado.| Biblioteca(s): Embrapa Solos. |
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| 6. | | VILLARREAL NUNEZ, J. E.; AMARAL SOBRINHO, N. M. B. do; KUNZMANN, M.; PALMIERI, F. Perdas de metais pesados por erosao influenciado pelo sistema de preparo do solo na microbacia de Caetes, Paty do Alferes, RJ. In: WORKSHOP NACIONAL DE AGRICULTURA SUSTENTAVEL EM REGIOES DE RELEVO ACIDENTADO, 3., 1997. Paty do Alferes, RJ. Anais... Rio de Janeiro: EMBRAPA-CNPS, 1998. CD-ROM Nao paginado.| Biblioteca(s): Embrapa Solos. |
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| 8. | | RIBEIRO, F. C. A.; LAURIA, D. da C.; SILVA, J. I. R.; LIMA, E. S. A.; AMARAL SOBRINHO, N. M. B. do; PEREZ, D. V. Baseline and quality reference values for natural radionuclides in soils of Rio de Janeiro State, Brazil. Revista Brasileira de Ciência do Solo, v. 42, article e0170146, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Solos. |
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| 9. | | BENAZZI, E. dos S.; MACHADO, H. M.; SOUSA, F. F. de; CORREIA, M. E. F.; AMARAL SOBRINHO, N. M. B. do. Ensaio de toxicidade aguda utilizando Eisenia fetida em solo artificial tratado com resíduos de perfuração de poço de petróleo. In: REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 10. | | BATISTA, I.; DIAS, F. de C.; AQUINO, A. M. de; MAZUR, N.; AMARAL SOBRINHO, N. M. B. do; OLIVEIRA, C. de. Resposta da macrofauna edáfica à aplicação de resíduos industriais em um planossolo. Seropédica: Embrapa Agrobiologia, 2006. 4 p. Trabalho apresentado na XXVII Reunião Brasileira de Fertilidade do Solo e Nutrição de Plantas, XI Reunião Brasileira sobre Micorrizas, IX Simpósio Brasileiro de Microbiologia do Solo; VI Reunião Brasileira de Biologia do Solo, Bonito/MS,...| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | LIMA, E. S. A.; MATOS, T. de S.; PINHEIRO, H. S. K.; GUIMARÃES, L. D. D.; PEREZ, D. V.; AMARAL SOBRINHO, N. M. B. do. Soil heavy metal content on the hillslope region of Rio de Janeiro, Brazil: reference values. Environmental Monitoring and Assessment, v. 190, article 364, Jun. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 12. | | RIBEIRO, F. C. A.; SILVA, J. I. R.; LIMA, E. S. A.; AMARAL SOBRINHO, N. M. B. do; PEREZ, D. V.; LAURIA, D. da C. Natural radioactivity in soils of the state of Rio de Janeiro (Brazil): radiological characterization and relationships to geological formation, soil types and soil properties. Journal of Environmental Radioactivity, v. 182, p. 34-43, Feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 13. | | MATTOS, A. G. de; LIMA, E. S. A.; AMARAL SOBRINHO, N. M. B. do; ZOFFOLI, H. J. de O.; PEREZ, D. V. Multivariate analyses to establish reference values for soils in Médio Paraíba, state of Rio de Janeiro, Brazil. Revista Ciência Agronômica, v. 49, n. 1, p. 1-10, jan./mar. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 15 | |
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